WormBase Tree Display for RNAi: WBRNAi00070114
expand all nodes | collapse all nodes | view schema
WBRNAi00070114 | Homol | Homol_homol | F53H2:RNAi | |
---|---|---|---|---|
Sequence_info | PCR_product | sjj_F53H2.1 | ||
Experiment | Laboratory | JF | ||
Date | 25 Jun 2007 00:00:00 | |||
Genotype | mtl-2(gk125) | |||
Treatment | Synchronized L1 larva were put on standard NGM plates with E. coli OP50 as food for approximately 31 h and allowed to develop into L4 larva. About 25 early L4 nematodes were then transferred to each well of a 6-well NGM plate, containing 1 mM IPTG and 25 mcg/ml carbenicillin, seeded with different RNAi expressing bacteria. Plates were then incubated at 20C for approximately 40 h. After the initial feeding period, 2 gravid adults from each well were then transferred to test plates containing RNAi bacteria and either 0 or 100 mcM cadmium. Each exposure was performed in triplicate. Control RNAi (empty vector) exposures were also performed for each cadmium treatment. Adults were allowed to lay eggs for approximately 12 h before removal. F1 nematodes were then grown on test plates for three additional days at 20C. At the end of the 3 day growth period, F1 nematodes were washed off the 6-well plates using M9 buffer and transferred onto 96-well plates. Body size measurements, as represented by nematode body length (time of flight (TOF)) were measured using the COPAS Biosort. | |||
Temperature | 20 | |||
Delivered_by | Bacterial_feeding | |||
Inhibits (3) | ||||
Species | Caenorhabditis elegans | |||
Reference | WBPaper00030811 | |||
Phenotype | WBPhenotype:0000031 | Remark | Difference between normalized mean body lengths of RNAi-treated animals exposed and not exposed to cadmium = 0.37 | |
WBPhenotype:0001655 | Remark | Strong protective effects against cadmium toxicity. | ||
Method | RNAi |