WormBase Tree Display for RNAi: WBRNAi00070083
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| WBRNAi00070083 | Homol | Homol_homol | Y105C5A:RNAi | ||
|---|---|---|---|---|---|
| C08E3:RNAi | |||||
| Sequence_info | PCR_product | sjj_C08E3.1 | |||
| Experiment | Laboratory | JF | |||
| Date | 25 Jun 2007 00:00:00 | ||||
| Genotype | mtl-2(gk125) | ||||
| Treatment | Synchronized L1 larva were put on standard NGM plates with E. coli OP50 as food for approximately 31 h and allowed to develop into L4 larva. About 25 early L4 nematodes were then transferred to each well of a 6-well NGM plate, containing 1 mM IPTG and 25 mcg/ml carbenicillin, seeded with different RNAi expressing bacteria. Plates were then incubated at 20C for approximately 40 h. After the initial feeding period, 2 gravid adults from each well were then transferred to test plates containing RNAi bacteria and either 0, 50, 100, or 200 mcM cadmium. Control RNAi (empty vector) exposures were also performed for each cadmium treatment. Adults were allowed to lay eggs for approximately 12 h before removal. F1 nematodes were then grown on test plates for three additional days at 20C. | ||||
| Temperature | 20 | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | Y105C5A.12 | Inferred_automatically | RNAi_primary | |
| C08E3.1 | Inferred_automatically | RNAi_primary | |||
| C08E3.13 | Inferred_automatically | RNAi_primary | |||
| Gene | WBGene00015593 | Inferred_automatically | RNAi_primary | ||
| WBGene00015605 | Inferred_automatically | RNAi_primary | |||
| WBGene00013636 | Inferred_automatically | RNAi_primary | |||
| Transcript | C08E3.1.1 | Inferred_automatically | RNAi_primary | ||
| Y105C5A.12.1 | Inferred_automatically | RNAi_primary | |||
| C08E3.13.1 | Inferred_automatically | RNAi_primary | |||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00030811 | ||||
| Phenotype_not_observed | WBPhenotype:0001655 | ||||
| Method | RNAi |
