WormBase Tree Display for RNAi: WBRNAi00066641
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WBRNAi00066641 | Homol | Homol_homol | C08F1:RNAi | |
---|---|---|---|---|
Sequence_info | PCR_product | sjj_C08F1.5 | ||
Experiment | Laboratory | AQ | ||
Date | 13 Jun 2005 00:00:00 | |||
Genotype | rrf-3(pk1426) | |||
Treatment | Three L4 animals were placed onto the RNAi plate, and transferred to a freshly induced plate after 24 h. Nicotine sensitivity of the progeny was scored 96 h later. For candidates causing nicotine sensitivity phenotypes, the RNAi experiment was repeated to verify the finding. | |||
Delivered_by | Bacterial_feeding | |||
Inhibits (3) | ||||
Species | Caenorhabditis elegans | |||
Reference | WBPaper00026635 | |||
Phenotype_not_observed | WBPhenotype:0001573 | Remark | Nicotine sensitivity assays: A total of 30 young adult animals were transferred to NGM plates containing 0.425 or 0.5% (v/v) (-)-nicotine, or 1 or 0.2mM levamisole. Paralysis was followed by visual inspection every 15 minutes and defined as lack of movement in response to prodding. | |
Method | RNAi |