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WormBase Tree Display for RNAi: WBRNAi00066532

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Name Class

WBRNAi00066532HomolHomol_homolW02A2:RNAi
Sequence_infoPCR_productsjj_W02A2.5
ExperimentLaboratoryAQ
Date13 Jun 2005 00:00:00
Genotyperrf-3(pk1426)
TreatmentThree L4 animals were placed onto the RNAi plate, and transferred to a freshly induced plate after 24 h. Nicotine sensitivity of the progeny was scored 96 h later. For candidates causing nicotine sensitivity phenotypes, the RNAi experiment was repeated to verify the finding.
Delivered_byBacterial_feeding
InhibitsPredicted_geneW02A2.5Inferred_automaticallyRNAi_primary
GeneWBGene00012190Inferred_automaticallyRNAi_primary
TranscriptW02A2.5.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00026635
PhenotypeWBPhenotype:0001202RemarkNicotine sensitivity assays: A total of 30 young adult animals were transferred to NGM plates containing 0.425 or 0.5% (v/v) (-)-nicotine, or 1 or 0.2mM levamisole. Paralysis was followed by visual inspection every 15 minutes and defined as lack of movement in response to prodding.
MethodRNAi