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WormBase Tree Display for RNAi: WBRNAi00065107

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Name Class

WBRNAi00065107HomolHomol_homolT19D12:RNAi
Sequence_infoPCR_productsjj_T19D12.4
ExperimentLaboratoryWE
Date12 Sep 2006 00:00:00
Genotypeglp-4(bn2);rrf-3(pk1426)
TreatmentL4 worms were placed on NGM plates containing 1 mM IPTG and preseeded with the designated RNAi clones. After 48 h of feeding on dsRNA-expressing bacteria, worms were washed off plates and transferred to NG plates containing Pseudomonas aeruginosa strain PA14.
Temperature25
Delivered_byBacterial_feeding
InhibitsPredicted_geneT19D12.4bInferred_automaticallyRNAi_primary
T19D12.4aInferred_automaticallyRNAi_primary
GeneWBGene00020579Inferred_automaticallyRNAi_primary
TranscriptT19D12.4b.1Inferred_automaticallyRNAi_primary
T19D12.4a.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00028482
PhenotypeWBPhenotype:0001013
RemarkTo avoid confounding effects of progeny in killing assays, used sterile strain glp-4(bn2);rrf-3(pk1426), which lacks the germline altogether (glp-4) and is more sensitive to RNAi (rrf-3). Pseudomonas aeruginosa strain used was the clinical isolate PA14.
MethodRNAi