WormBase Tree Display for RNAi: WBRNAi00065089

WBRNAi00065089 Homol: Homol_homol: Y70C5C:RNAi
  Sequence_info: PCR_product: sjj_Y70C5C.6
  Experiment: Laboratory: WE
    Date: 12 Sep 2006 00:00:00
    Genotype: glp-4(bn2);rrf-3(pk1426)
    Treatment: L4 worms were placed on NGM plates containing 1 mM IPTG and preseeded with the designated RNAi clones. After 48 h of feeding on dsRNA-expressing bacteria, worms were washed off plates and transferred to NG plates containing Pseudomonas aeruginosa strain PA14.
    Temperature: 25
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: Y70C5C.6 Inferred_automatically: RNAi_primary
    Gene: WBGene00003702 Inferred_automatically: RNAi_primary
    Transcript: Y70C5C.6.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Reference: WBPaper00028482
  Phenotype: WBPhenotype:0001013
  Remark: To avoid confounding effects of progeny in killing assays, used sterile strain glp-4(bn2);rrf-3(pk1426), which lacks the germline altogether (glp-4) and is more sensitive to RNAi (rrf-3). Pseudomonas aeruginosa strain used was the clinical isolate PA14.
  Method: RNAi