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WormBase Tree Display for RNAi: WBRNAi00064873

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Name Class

WBRNAi00064873HomolHomol_homolM03F8:RNAi
Sequence_infoPCR_productsjj_M03F8.3
ExperimentLaboratoryNL
Date13 Feb 2004 00:00:00
Genotypeunc-54::POLYQ(Q35)-YFP
Temperature23
Delivered_byBacterial_feeding
InhibitsPredicted_geneM03F8.3aInferred_automaticallyRNAi_primary
M03F8.3bInferred_automaticallyRNAi_primary
GeneWBGene00019762Inferred_automaticallyRNAi_primary
TranscriptM03F8.3b.1Inferred_automaticallyRNAi_primary
M03F8.3a.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00006510
PhenotypeWBPhenotype:0000679RemarkRNAi caused early onset of foci formation (polyglutamine-YFP aggregation)
EQ_annotationsLife_stageWBls:0000041PATO:0000460
WBPhenotype:0001405RemarkRNAi caused early onset of foci formation (polyglutamine-YFP aggregation)
EQ_annotationsLife_stageWBls:0000041PATO:0000460
RemarkIn strains expressing unc-54::POLYQ(Q35)-YFP, a diffuse YFP staining pattern is seen in all expressing cells from the moment they hatch through young adulthood. Onset of aggregation is invariable and occurs after the animals reach adulthood at the fifth day after hatching. L1 larva were feed RNAi bacteria in liquid culture and animals were assayed for fluorescent foci three days post-L1.
MethodRNAi