WormBase Tree Display for RNAi: WBRNAi00064525
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| WBRNAi00064525 | Homol | Homol_homol | C04A2:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | PCR_product | sjj_C04A2.2 | |||
| Experiment | Laboratory | ANF | |||
| Date | 16 Jul 2006 00:00:00 | ||||
| Strain | WBStrain00022494 | ||||
| Treatment | 37 mutant worm strains were screened with an RNAi library consisting of 1,860 bacterial feeding strains targeting 1,744 different worm genes. All of the RNAi clones that gave a stronger RNAi phenotype in at least one worm strain compared with wild-type worms were retested in all of the 37 query strains (in duplicate) in order to reduce the rate of false negatives. All interactions from this second round of screening were retested, and interactions observed in at least two of four repeats were considered as positives, giving a total of 377 interactions between query strains and library RNAi clones (equivalent to 349 interactions between 162 genes) | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene (18) | ||||
| Gene | WBGene00001194 | Inferred_automatically | RNAi_primary | ||
| Transcript (24) | |||||
| Species | Caenorhabditis elegans | ||||
| Interaction | WBInteraction000006052 | ||||
| Reference | WBPaper00027756 | ||||
| Phenotype | WBPhenotype:0000050 | ||||
| WBPhenotype:0000688 | |||||
| Remark | Genetic enhancer interactions are identified by comparing RNAi phenotypes of library genes in wild-type animals with those seen in mutant worms carrying a defined lesion in a single query gene; a genetic interaction between a query gene and a library gene is observed if the phenotype of the combined perturbation is greater than the sum of the two individual perturbations. | ||||
| Method | RNAi |
