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WormBase Tree Display for RNAi: WBRNAi00002732

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Name Class

WBRNAi00002732History_nameTH:340f10
HomolHomol_homolC30C11:RNAi
Sequence_infoPCR_productTH:340f10
ExperimentLaboratoryTH
Date16 Nov 2000 00:00:00
InhibitsPredicted_geneC30C11.2Inferred_automaticallyRNAi_primary
GeneWBGene00004460Inferred_automaticallyRNAi_primary
TranscriptC30C11.2.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00004403
PhenotypeWBPhenotype:0000050
RemarkPhenotype comment -- Confirmed with independent dsRNA (C30C11.2-RNA2; similar phenotype).
DIC phenotype -- In most embryos (e.g. movie1), the male and female pronuclei never become visible, and everything seems to be arrested. Some embryos progressed to the one cell stage (e.g. movie2): in these cases, there is a sriking delay during mitosis (over 20 min between breakdown of the pronuclear envelopes and cleavage furrow ingression, compared to the usual approximately 2 min in wild-type); earlier events in the one cell stage may be slow as well.
DIC phenotype comment -- Staining of fixed embryos with DNA dye shows that fertilization took place, and suggests that at least half of the embryos are arrested during meiotic divisions.
All embryos dead
MethodRNAi