WormBase Tree Display for Picture: WBPicture0000015215
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| WBPicture0000015215 | Description | Figure 1. A. Schematic of six chromosomes in wildtype worms drawn to scale, as well as in backgrounds with chromosomal rearrangements, mIn1/+, hIn1/+, meDf2 and mnT12. Arrows indicate the right end of the chromosomes and dashed gray lines indicate portions of chromosomes inverted in mIn1/+ and hIn1/+. meDf2 is a terminal or near-terminal deletion of the left portion of the X chromosome, with some ambiguity regarding its breakpoint (shaded portion), and mnT12 is a fusion between chromosomes X and IV. Representative images of wildtype (B), mIn1/+ (C), and hIn1/+ (D) hermaphrodite germlines stained with DAPI (top) and antibodies against DSB-1 (middle) and PCH-2 (bottom). E. Fraction of total meiotic nuclei in the germline stained with DSB-1 and PCH-2 in wildtype, mIn1/+, and hIn1/+ germlines (n=5 per genotype). No significant difference (ns) is observed between wildtype, mIn1/+, and hIn1/+. Representative images of meDf2 (F) and mnT12 hermaphrodite germlines (G) stained with DAPI (top) and antibodies against DSB-1 (middle) and PCH-2 (bottom). H. Fraction of total meiotic nuclei in the germline stained with DSB-1 and PCH-2 in wildtype, meDf2, and mnT12 germlines (n=4 for wildtype and n=5 for meDf2 and mnT12). I. Representative images of meiotic nuclei stained with DAPI (magenta) and GFP::COSA-1 (green). The nucleus in mnT12 has six GFP::COSA-1 foci while the nucleus in mnT12;pch-2 has five. Scale bar indicates 2 microns. J. Histogram representing the fraction of meiotic nuclei with four, five, six, and seven GFP::COSA-1 foci in mnT12 (n=483 nuclei) and mnT12;pch-2 mutants (n=446 nuclei). In all graphs, error bars indicate standard deviation and ** indicates a p value < 0.001. Unless otherwise stated, scale bars in images indicate 8 microns. | |||
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| Name | 434d881b72f8262a6f716d1623e4460e.jpg | ||||
| Acknowledgment | Template | Reprinted from <Journal_URL> | |||
| Publication_year | 2023 | ||||
| Article_URL | DOI | id | 10.17912/micropub.biology.000839 | ||
| Journal_URL | microPublicationBiology | ||||
| Publisher_URL | Micropublication | ||||
| Reference | WBPaper00065385 |
