WormBase Tree Display for Picture: WBPicture0000015106

WBPicture0000015106 Description: Figure 3. Nucleic acid binding transporter SID-2 is specifically enriched in the male- specific ciliated EV-releasing neurons (EVNs) and is shed in the form of EVs to the environment(A) Validation protocol for putative ciliary EV cargo. (B and C) EV release of SID-2::mScarlet and PKD- 2::GFP from cilia of CEM neuron of the head (B) and RnB neuron of the tail (C). (C) shows ray #7 in the process of shedding SID-2::mScarlet as evidenced by continuous fluorescence signal spanning the ciliary base, ciliary shaft, and the outside cloud of EVs. Other clouds on (C) are not labeled as EV release events because it was not clear from which cilium they originated. Resolution of the imaging technique was 140 nm in lateral di- rection and 400 nm in axial direction; thus, smaller EVs appear as clouds rather than individual EVs. Areas of intense inherent autofluorescence of the male tail are shaded with gray and labeled as au- toflu. Images show 3D renderings: on merged im- ages green and magenta colors represent different reporters, on images showing individual channels colors code for Z-depth-3.5 mm for (B) and 6.5 mm for (C).Ci, cilia; CEM, cephalic male neuron; HOB, hook neuron type B; RnB, ray neuron type B. Black and white maximum intensity projections of (B) and (C) and other data related to SID- 2::mScarlet expression are included in Figure S3.
  Name: INNA_FIG_3.jpg
  Depict: Expr_pattern: Expr15979
      Expr15980
    Anatomy: WBbt:0004761
      WBbt:0005246
      WBbt:0005772
      WBbt:0006973
    Cellular_component: GO:0043025
      GO:0045177
      GO:0097730
  Acknowledgment: Template: Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
    Publication_year: 2022
    Article_URL: DOI id 10.1016/j.cub.2022.03.005
    Journal_URL: CurrentBiology
    Publisher_URL: Elsevier
  Reference: WBPaper00062669