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WBPicture0000014803DescriptionFig 7. Dynamic expression of the HS copolymerase during development. A. rib-1 transcriptional (Prib-1::gfp) and functionaltranslational (Prib-1::rib-1::venus) reporters display similar expression patterns. Expression of rib-1 is observed in numerous cell types,including in the pharynx, hypodermal cells, neurons (arrows), the enteric muscle and the anal depressor. The RIB-1::Venus signal is punctate, consistent with a Golgi localization. Scale bars: 50 um in top panels of entire worms, and 5 um in lower panels. B. Larval andadult expression of rib-1. (i) Throughout larval and adult stages, the expression of Prib-1::gfp is observed in the pharynx, including in thepharyngeal-intestinal valve (ii, closed arrowhead) and muscles of the anterior and terminal pharyngeal bulbs (iii, pharyngeal muscle pm6);in the enteric muscle (open arrowhead, see A); in the ventral nerve cord (arrows, iv, see A); in the dorsal nerve cord (v); in spermathecae(asterisks, vi, seen enlarged by an oocyte in vii); and in uterine muscles (viii). Scale bars, 50 um in panels depicting entire worms, and5 um in the rest of the panels. C. Embryonic expression of rib-1. Views of comma stage embryos in GFP epifluorescence and DIC. Thetranscriptional reporter Prib-1::gfp is predominantly expressed in lateral and ventral epidermal cells that undergo major morphogeneticmovements during dorsal intercalation and ventral enclosure of the embryo. Scale bar, 5 um. D. rib-1 is expressed during vulva development. Views of the developing vulva in fourth larval stage animals by GFP and DIC epifluorescence. Prib-1::gfp is expressed in epidermalcells of the developing vulva at a time when these cells undergo major rearrangements. Scale bar, 10 um.
NameFig7.jpg
CropCrop_pictureWBPicture0000014804
DepictExpr_patternExpr13901
Anatomy (24)
AcknowledgmentTemplateReprinted from <Journal_URL>, <Article_URL>. <Publisher_URL> <Publication_year>.
Publication_year2017
Article_URLDOIid10.1371/journal.pgen.1006525
Journal_URLPLoSGenetics
Publisher_URLPLoS
ReferenceWBPaper00050624