WormBase Tree Display for Picture: WBPicture0000014430

WBPicture0000014430 Description: FIGURE 2: Tubulin isoform expression in PLM. (A) Expression of 5099 C. elegans genes (5086 protein coding and 13 ncRNA coding) that exhibited above-background expression in PLM (Materials andMethods and Supplemental Table S4). Gene expression, measured inTPM, vs. expression in PLM relative to expression in whole larvae. Each gray or red dot represents a single gene expressed in PLM; red dots are 1060 housekeeping genes (Schwarz et al., 2012). Genes encoding eight tubulins are highlighted as larger squares and those encoding 12 nontubulin mec genes are shown as larger green triangles. The dashed vertical line highlights 100-fold more transcripts in PLM than in whole larvae. (B) Coexpression of alpha-tubulin and beta-tubulin TagRFP-T protein fusions and uIs31 TRN::GFP transcriptional fusion. Left, uIs31(mec-17p::gfp) showing PLM. Center, TagRFP-T tubulin fusion protein. Right, composite image. TagRFP-T was false colored as magenta for enhanced visibility. Expression of isoforms inTRNs is seen as white in the composite overlap of uIs31 marker and TagRFP-T::Tubulin. Images are oriented so that the anterior of the animal is on the left and the ventral side is on the bottom. Scale bar, 50 um.
  Name: Fig2.jpg
  Crop: Crop_picture: WBPicture0000014431
  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article <Article_URL>. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 2016
    Article_URL: DOI id 10.1091/mbc.E16-06-0473
    Journal_URL: MolecularBiologyoftheCell
    Publisher_URL: TheAmericanSocietyForCellBiology
  Reference: WBPaper00050344