WormBase Tree Display for Picture: WBPicture0000013334

WBPicture0000013334 Description: Figure 4. Epistasis Analysis of Muscle Attachment Assembly(A) Localization of UNC-52/perlecan, PAT-3/β-integrin, UNC-112, PAT-4/ILK, DEB-1/vinculin, and UNC-89 in wild-type (1-6) and pat-6(st561) (1'-6') in 530 min embryos. Arrows in all panels except for 6' indicate body wall muscle attachments. Arrows in 6' indicate clumps of UNC-89 protein located in the cytoplasm of body wall muscle cells. All proteins visualized by antibody staining except for UNC-112, which was visualized using a functional unc-112::gfp transgene.(B) Localization of PAT-6/actopaxin in wild-type, unc-52(st549), pat-3(st552), unc-112(gk1), pat-4(st551), and deb-1(st555) embryos (1-6). Arrows indicate regions of anti-PAT-6/actopaxin staining. PAT-6/actopaxin is properly recruited to muscle attachments in the wild-type (1) and deb-1 (6) embryos, but not in the unc-52(st549), pat-3(st552), unc-112(gk1), or pat-4(st551) embryos (2-5). The embryos were also stained with monoclonal antibody MH27 to hypodermal cell junctions, which appear as a grid-like pattern and are indicated by arrowheads in panel 5. Scale bar (A1), 5 μm.
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  Acknowledgment: Template: Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
    Publication_year: 2003
    Article_URL: DOI id 10.1016/S0960-9822(03)00372-5
    Journal_URL: CurrentBiology
    Publisher_URL: Elsevier
  Reference: WBPaper00005915