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WBPicture0000013316DescriptionFigure 5. Expression of each fog-3 gene is correlated with spermatogenesis. (A) Developmental Northern blot of total RNA from populations of C. briggsae XX animals. M, mixed ages; L1 to L4, four larval stages; yA, young adults; A, adults. We used a probe for 18s ribosomal RNA on the same blot to control for variations in loading. The probes are described in materials and methods. (B) Quantitative RT-PCR analysis of fog-3 expression in C. remanei. RNA was prepared from single worms of the indicated ages and used for RT-PCR analysis of fog-3 expression. The number of cycles was set so that a twofold dilution of the male samples yielded a corresponding decrease in the intensity of the resulting band.
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AcknowledgmentTemplateWormBase thanks the journal <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>
Publication_year2001
Article_URLDOIid10.1093/genetics/158.4.1513
Journal_URLGenetics
Publisher_URLGSA
ReferenceWBPaper00004832