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WBPicture0000012680DescriptionFigure 4. Expression Pattern and Site-of-Function of CYSL-1 (A) Fluorescence and Nomarski images showing the expression pattern of cysl-1 as visualized by the integrated transcriptional GFP reporter nIs500, which harbors a 2.8 kb promoter of cysl-1 fused to GFP. Head neurons are indicated by the arrow, and tail neurons are indicated by the arrowhead. (B) Enlarged view of fluorescence micrograph showing AVM and BDU neurons. Scale bars: 25 um. (C) Confocal microscopic view of pharyngeal (I1 and M2 indicated by arrows) and head neurons. (D and E) Expression patterns of cysl-1 as visualized by the extrachromasomal array nEx1838 with a translational GFP reporter harboring the promoter and genomic coding regions of cysl-1 fused in-frame to GFP. (F and G) Rescue of cysl-1(n5515) phenotypes by neuronal expression of cysl-1(+) cDNA driven by the ric-19 promoter. (H) The unc-14 promoter-driven neuronal activation of HIF-1 causes defects in the O2-ON response of egl-9(sa307); hif-1(ia4) double mutants. (I) The dpy-7 promoter-driven hypodermal activation of HIF-1 does not cause defects in the O2-ON response of egl-9; hif-1 double mutants.
NameFigD.jpg
CropCrop_pictureWBPicture0000012681
AcknowledgmentTemplateReprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
Publication_year2012
Article_URLDOIid10.1016/j.neuron.2011.12.037
Journal_URLNeuron
Publisher_URLElsevier
ReferenceWBPaper00040881