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WBPicture0000012616 | Description | Figure 3. The synMuv genes prevent widespread ectopic expression of lin-3 mRNA. FISH of lin-3 mRNA in late L2 to early L3 animals. Each dot represents a single mRNA molecule [42]. lin-3 mRNAs are shown in red, and 49,6-diamidino-2-phenylindole (DAPI) staining of nuclei is shown in blue. The images shown are maximum intensity projections of a z-stack of images. The anchor cell (AC) is indicated by an arrowhead in each panel. (A) Wild type. lin-3 mRNA is expressed in the pharynx, anchor cell, and germline and is tightly repressed elsewhere. (B) lin-15A(n767). There is a low level of ectopic lin-3 expression, with approximately 60 ectopic copies of lin-3 mRNA seen outside the pharynx, anchor cell, and germline. (C) lin- 15B(n744). There is a very low level of ectopic lin-3 expression, with approximately 10 ectopic copies of lin-3 mRNA seen outside the pharynx, anchor cell, and germline. The large bright spot at the edge of the head is outside the animal. (D) lin-15AB(e1763). lin-3 is ectopically expressed throughout the animal, with approximately 900 ectopic copies of lin-3 mRNA seen outside the pharynx, anchor cell, and germline. | |||
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Name | FigH.jpg | ||||
Crop | Crop_picture | WBPicture0000012617 | |||
Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>. <Publisher_URL> <Publication_year>. | |||
Publication_year | 2011 | ||||
Article_URL | DOI | id | 10.1371/journal.pgen.1002418 | ||
Journal_URL | PLoSGenetics | ||||
Publisher_URL | PLoS | ||||
Reference | WBPaper00040630 |