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WBPicture0000012573 | Description | Figure 2. PCP genes act autonomously in VC4 and VC5 and non-autonomously from epithelial cells to inhibit neurite growth. (A) Schematic representation of genomic rescuing constructs and GFP transcriptional reporters used in this study. (B-D) prkl-1 (B), vang-1 (C), and dsh-1 (D) transcriptional activity (GFP) is present in VC neurons during the period of VC neurite extension (L4 stage) and in vulval cells (arrowhead in B). Expression is also seen in a subset of ventral cord neurons and uterine cells. Due to widespread expression of vang-1 and dsh-1 in neurons, the VC1-6 and vulval cell reporter Plin-11::RFP was used to unambiguously identify VC neurons (yellow in merged image). Scale bars, 20 um. (E) Cell-specific rescue experiments using functional GFP-tagged proteins suggest that prkl-1 acts autonomously in VC neurons, whereas vang-1 and dsh-1 act both autonomously in VC neurons and non-autonomously to restrict neurite emergence. Representative transgenic lines are shown, but at least two independent lines were examined per experiment. All lines carry a cyIs4 reporter. Error bars represent standard error of proportion; n.100. *p,0.001, x2 (compared to non-transgene expressing mutants). | |||
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Name | FigF.jpg | ||||
Crop | Crop_picture | WBPicture0000012574 | |||
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Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>. <Publisher_URL> <Publication_year>. | |||
Publication_year | 2011 | ||||
Article_URL | DOI | id | 10.1371/journal.pgen.1002257 | ||
Journal_URL | PLoSGenetics | ||||
Publisher_URL | PLoS | ||||
Reference | WBPaper00040178 |