WormBase Tree Display for Picture: WBPicture0000012546

WBPicture0000012546 Description: Fig. 1. Tissue-specific expression of klf-3 in the intestine. (a) A translational fusion construct was created by the fusion of a 2-kb promoter region upstream of the klf-3b ATG and its full coding sequence consisting of six exons in-frame with the gfp reporter (pHZ145). (b) A 1-kb promoter region upstream of klf-1 ATG and the full-length cDNA of fat-7 were fused inframe with the gfp reporter (pHZ343). Exons are indicated as shaded black boxes, and gray boxes indicate 5' and 3' untranslated regions. Promoters and introns between exons are indicated by a continuous line. (c) Images of klf-3b::gfp expression in the adult worm. Transgenic lines of C. elegans carrying the pHZ145 construct for the klf-3::gfp fusion gene were generated as described previously. As shown, klf-3b::gfp expression (green fluorescent) is seen in intestinal segments covering the midbody and the tail region of a (c) C. elegans larva and a (d) young adult worm, but is absent from the gonadal region (gray area adjacent to green fluorescent are) and the vulval region (arrow). For clarity, GFP expression image is merged with differential interference contrast images. Transgenic worms were observed and paragraphed using an Axioskop 2 Plus fluorescent microscope with appropriate filter sets (magnification of 400).
  Name: FigA.jpg
  Depict: Expr_pattern: Expr9597
    Anatomy: WBbt:0005772
  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 2011
    Article_URL: DOI id 10.1016/j.jmb.2011.06.011
    Journal_URL: JournalofMolecularBiology
    Publisher_URL: Elsevier
  Reference: WBPaper00039810