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WBPicture0000012374 | Description | Figure 5. Posterior Localization of LIN-17 in PLM(A and B) Fluorescence images and diagrams of PLM in (A) wild-type and (B) lin-44(n1792) animals expressing the kyEx838 transgene (mec-7::lin-17::mRFP1) and zdIs5 (mec-4::GFP).(C) Fluorescence images and diagrams of PLM in wild-type animals expressing the kyEx1235 transgene (mec-7::lin-17::mRFP1) and zdIs5 (mec-4::GFP).(D) Quantitation of LIN-17 localization. Fluorescence intensity ratio of the anterior/posterior processes in wild-type (n = 28) and lin-44 animals (n =10) expressing the kyEx838 transgene (mec-7::lin-17::mRFP1), and in wild-type (n = 13) animals with reversed PLM neurons due to overexpression of the kyEx1235 transgene (mec-7::lin-17::mRFP1). *, different from wild-type animals, p < 0.05, Bonferroni t test for proportion.(E) Fluorescence image and diagram of a PLM defect caused by LIN-17 overexpression. kyEx939 (mec-7::lin-17 at 5 ng/ml) in an animal expressingmec-4::GFP. The PLM is reversed on the A/P axis. In this animal, additional processes extend from the PLM cell body in random directions(5%-10% penetrance, defect arises mostly after L1 stage).(F) Quantitation of the PLM polarity defect (reversal) resulting from LIN-17 overexpression. Different transgenic animals carrying mec-7::lin-17injected at different concentrations. kyEx738 (0.1 ng/ml); kyEx837 and kyEx840 (1 ng/ml); kyEx939 and kyEx938 (5 ng/ml).Error bars indicate standard error of proportion. | |||
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Name | F5.jpg | ||||
Crop | Crop_picture | WBPicture0000012375 | |||
Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
Publication_year | 2006 | ||||
Article_URL | DOI | id | 10.1016/j.devcel.2006.01.013 | ||
Journal_URL | DevelopmentalCell | ||||
Publisher_URL | Elsevier | ||||
Reference | WBPaper00027141 |