WormBase Tree Display for Picture: WBPicture0000012274
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| WBPicture0000012274 | Description | FIG. 3. Expression of ptp-ferm::ptp-ferm-gfp transgene in C.elegans. (A) A schematic drawing of ptp-ferm::ptp-ferm-gfp chimeric minigene. A ptp-ferm genomic fragment containing exon 1-3 (nt5880-1232) was fused to a cDNA encoding PTP-FERM (type I)and a GFP minigene that has introns followed by the 3 untranslated region of unc-54. The construct is designed to express a fusionprotein, PTP-FERM-GFP, under the regulation of PTP-FERM promoter. (B) Fluorescence micrograph of PTP-FERM-GFP expression. Representative results from four independent worm lines that have ptp-ferm::ptp-ferm-gfp as an extrachromosomal array are shown. The small arrows show cell bodies and the large arrows show nerve processes. b is a confocal microscopic image of a neuron. Images d and f are bright field images of c and e, respectively.N, neuron on the lateral side of the worm; V, vulva; VN, ventral nerve cord. Scale bars, 5um. (C) Immunoblotting study of PTP-FERM-GFP. Lysates were prepared from N2 worms (lane 1)or a line that has ptp-ferm::ptp-ferm-gfp as an extrachromosomal array (lane 2) and analyzed by immunoblotting using anti-GFP antibody. | |||
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| Name | FigE.jpg | ||||
| Depict | Expr_pattern | Expr10816 | |||
| Anatomy | WBbt:0003679 | ||||
| WBbt:0005829 | |||||
| WBbt:0006748 | |||||
| Cellular_component | GO:0030424 | ||||
| GO:0043025 | |||||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
| Publication_year | 2002 | ||||
| Article_URL | DOI | id | 10.1006/bbrc.2002.6613 | ||
| Journal_URL | BiochemicalandBiophysicalResearchCommunications | ||||
| Publisher_URL | Elsevier | ||||
| Reference | WBPaper00005184 |
