WormBase Tree Display for Picture: WBPicture0000011811

WBPicture0000011811 Description: FIGURE 3. (A) (Panels A, B) Expression pattern of xrn-1GFP. GFP fluorescence pattern of animals carrying a rol-6 marker and a GFP reporter fused to the second exon of xrn-1, expressed as a nonintegrated extrachromosomal array. (B) Effect of xrn-1 on RNA interference. Panels A and B: Expression of an integrated histone GFP marker in the nuclei of oocytes (A) and embryos (B) in the germline of wild-type hermaphrodites. Panel C: histoneGFP hermaphrodites injected with double-stranded GFP RNA showing complete silencing of GFP expression, 48 h postinjection. Panel D: Co-injection of dcr-1 and GFP double-stranded RNA into hermaphrodites showing faint fluorescence in oocytes. Panel E: Silencing of GFP expression in adult worms co-injected with double-stranded GFP RNA and double-stranded unc-22 RNA 48 h postinjection. Panel F: histoneGFP hermaphrodites co-injected with xrn-1 and GFP double-stranded RNA showing faint fluorescence in the oocyte nuclei of the germline 48 h after gonad injection. Panel G: Triple injection of double-stranded xrn-1, dcr-1, and GFP RNA into hermaphrodites results in faint fluorescence in oocytes in most worms. Panel H: Control triple injection of unc-22, mab-9, and GFP double-stranded RNA showing no fluorescence except for autofluorescence in the gut. Scale bar, 50 um.
  Name: FigC.jpg
  Depict: Expr_pattern: Expr2827
  Acknowledgment: Template: WormBase wishes to thank the journal <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 2004
    Article_URL: DOI id 10.1261/rna.2195504
    Journal_URL: RNA
    Publisher_URL: ColdSpringHarborLabPress
  Reference: WBPaper00006308