WormBase Tree Display for Picture: WBPicture0000011803

WBPicture0000011803 Description: Figure 2. elt-4 is expressed in all cells of the intestine plus nine cells in the posterior pharynx.(A) Schematic representation of the three reporter gene fusions used to determine the elt-4 expression pattern. At the top the elt-4/elt-2 locus is shown, with scale centered on the initiation codon of elt-4. The three constructs are: pJM401, in which 5.5 kb of the elt-4 5'-flanking region are fused to GFP immediately after the elt-4 ATG codon; pJM156, in which 6.8 kb of the elt-4 5'-flanking region plus the elt-4 coding region are fused to GFP immediately before the elt-4 termination codon; and pJM188, a construct whose 11.2-kb insert contains 4.5 kb of the elt-4 5'-flanking region, the entire elt-4 coding region, the region between elt-4 and elt-2 (which includes the elt-2 enhancer), and approximately half of the elt-2 coding region (but not including the elt-2 DNA-binding domain), into which a GFP coding sequence has been inserted immediately before the elt-4 termination codon. (B) GFP fluorescence observed in embryos transformed with pJM188. B1, differential interference contrast (DIC) optics; B2, GFP fluorescence. Two embryos are at the 1.5-fold stage and two embryos are at the 3-fold stage. GFP expression is detected in all gut nuclei. Fluorescent images represent a maximum point projection of an aligned stack of nine deconvolved images taken at focal planes spaced at 1-um intervals. Bar, 20 um. (C) GFP fluorescence observed in an L1 larva transformed with pJM188. C1, DIC; C2, GFP fluorescence. GFP expression is detected in all nuclei of the intestine plus nine nuclei in the posterior bulb of the pharynx (arrow). Bar, 20 um. (D) Pharynx of an adult worm transformed with pJM188. D1, DIC; D2, GFP fluorescence. Fluorescent images represent a maximum point projection of an aligned stack of 15 deconvolved images taken at focal planes spaced at 1-um intervals. A full through-focus series reveals nine expressing nuclei (see text). Bar, 10 um.
  Name: FigB.jpg
  Depict: Expr_pattern: Expr2705
    Anatomy: WBbt:0003681
      WBbt:0005772
  Acknowledgment: Template: WormBase thanks the journal <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>
    Publication_year: 2003
    Article_URL: DOI id 10.1093/genetics/165.2.575
    Journal_URL: Genetics
    Publisher_URL: GSA
  Reference: WBPaper00006178