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| WBPicture0000011725 | Description | Figure 2. Immunocytochemistry of HSP43. All animals orientated with anterior to the left unless otherwise mentioned. In all images, HSP43 staining is shown in green. (A) Young adult. HSP43 is concentrated in the vulva (v) and spermathecae (sp). (B) HSP43 expression in the adherens junctions of vulval epithelia (ventral view). HSP43 (B1) and MH27, which stains desmosomes (B2), are partially co-localized (B3). (C) Ventral view. The relative position of HSP43 in vulva (green) is compared to vulval muscle (red, arrows, phalloidin). (D) Overview of HSP43 staining in vulval region (adult, lateral view). Arrows indicate utse and 2 uv1 cells. (E1) H-shaped utse, ventro-lateral view. (E2) and (E3) uv1 cells, with adjacent DAPI-stained gonadal nuclei (shown in red). (F) Spermathecal expression of HSP43. (F1) HSP43 staining in the spermathecal cage (green) with DAPI-stained sperm nuclei (shown in blue). (F2) HSP43 (green) is co-localized with desmosomes (red, MH27), except in spermathecal valve (arrow). (F3) More lateral view of a spermathecal cage, with HSP43 staining in green and nuclei (DAPI) in red. (F4) HSP43 staining in spermathecal valve region (green) compared with valve-muscle staining (red, phalloidin); HSP43 labels both ends of the phalloidin pattern. (G1) and (G2) L2 showing staining of muscle in red (phalloidin, G1) and nuclei in blue (DAPI, G2). The pharyngeal-intestinal valve (left arrow, G1) and intestinal-anal valve (right arrow, G1) are labelled by HSP43. (H), HSP43 staining over body-wall muscle. (H1) L3 larva. The signal is more intense where muscle cells contact one another (arrows). (H2) magnified region of L3-larval muscle surface, showing punctate pattern of HSP43 staining forming columns running circumferentially over muscle cells, probably at sites of hypodermal contacts. Columns can be resolved as doublets of elongated spots (arrows). (I) and (J) Specificity of anti-HSP43. No HSP43 signal was seen in spermatheca (I) or vulva (J) when antiserum was pre-incubated with excess pure HSP43. DAPI staining (blue) shows location of nuclei (I1 and J1), and co-staining with MH27 (red, I2) or DM5.6 (red, J2) indicates efficient permeabilization of the animals. | |||
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| Name | FigB.jpg | ||||
| Depict | Expr_pattern | Expr8560 | |||
| Anatomy | WBbt:0003672 | ||||
| WBbt:0004510 | |||||
| WBbt:0005319 | |||||
| WBbt:0005753 | |||||
| WBbt:0005767 | |||||
| WBbt:0005813 | |||||
| WBbt:0006748 | |||||
| WBbt:0006789 | |||||
| WBbt:0006791 | |||||
| Cellular_component | GO:0005912 | ||||
| GO:0030057 | |||||
| Acknowledgment | Template | Reproduced with permission, from <Article_URL>, <Publication_year>, <Journall_URL>. Copyright <Publisher_name>. | |||
| Publication_year | 2000 | ||||
| Article_URL | DOI | id | 10.1042/0264-6021:3490409 | ||
| Journal_URL | BiochemicalJournal | ||||
| Publisher_URL | PortlandPressLTD | ||||
| Reference | WBPaper00004245 |
