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WBPicture0000011633DescriptionFigure 4.- Localization of let-858 gene products. (A and B) In situ analysis of let-858 mRNA in whole mount wild-type embryos. Embryos were hybridized to let-858 antisense probe visualized using alkaline-phosphatase-mediated detection. let-858 mRNA (purple color) appears uniformly localized in all stages of embryos; A. DAPI; B, alkaline phosphatase. (C-E) Antibody analysis of let-858 protein in whole mount wild-type embryos (C and D) and adult (E). FITC labeled secondary antibodies were used to locate specific binding of mouse anti-LET-858 antibodies. Arrow heads in C and D indicate mitotic cells; the inset in E is an enlargement of the pachytene-stage nuclei in the adult gonad.
NameFigB.jpg
DepictExpr_patternExpr1575
AcknowledgmentTemplateWormBase thanks the journal <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>
Publication_year1997
Article_URLDOIid10.1093/genetics/146.1.227
Journal_URLGenetics
Publisher_URLGSA
ReferenceWBPaper00002774