WormBase Tree Display for Picture: WBPicture0000011615

WBPicture0000011615 Description: Figure 3. daf-7p::gfp expression in ASI neurons. (A to C) Wild-type background. (A) L1 larva; (B) dauer larva induced by pheromone; (C) recovering dauer larva about 8 hours after transfer to food at 25°C. (D) daf-7(n696) mutant background. Daf-c dauer larva, 20°C. g indicates gut autofluorescence. Arrowhead in (B) indicates the expected position of ASI cell bodies. Bars, 10 μm. (A) and (D) are three-dimensional confocal images. Faint fluorescence is apparent in the ASI axons and dendrites. (E) Diagrammed positions of the pharynx and cell bodies of ASI and neighboring neurons used for reference (one side shown). (F) GFP expression during dauer entry and recovery at 25°C. Preparation of crude pheromone extract and induction of dauer formation were as described (3). An average of 70% dauer larvae were induced with 100 μl of pheromone extract. A culture without pheromone was used as a control. Pheromone-induced dauer larvae were transferred to fresh food for recovery. PD1 and PD2 are the post-dauer equivalent of L3 and L4, respectively. Data are means ± SD for three replicates. About 20 animals were examined for each replicate.
  Name: FigC.jpg
  Depict: Expr_pattern: Expr623
    Anatomy: WBbt:0003887
      WBbt:0003888
  Acknowledgment: Template: From <Article_URL>. Reprinted with permission from <Publisher_URL>. Readers may view, browse, and/or download material for temporary copying purposes only, provided these uses are for noncommercial personal purposes. Except as provided by law, this material may not be further reproduced, distributed, transmitted, modified, adapted, performed, displayed, published, or sold in whole or in part, without prior written permission from the publisher.
    Publication_year: 1996
    Article_URL: DOI id 10.1126/science.274.5291.1389
    Journal_URL: Science
    Publisher_URL: AAAS
  Reference: WBPaper00002600