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WBPicture0000011612 | Description | Figure 8. Detection of emo-1 transcripts by in situ hybridization. Dissected gonads hybridized with a single-stranded anti-sense emo-1 DNA probe are shown visualized with Nomarski optics (A-D) or with epifluorescence to show DAPI staining of DNA (E). In both wild-type XX hermaphrodite (A) and XO male (B) gonads, pachytene-stage germ cells show strong signal while distal mitotic cells show little or no signal. Female XX germ cells maintain emo-1 RNA accumulation as they form oocytes, whereas male XO cells lose emo-1 transcript. Stronger emo-1 signal is seen in the hermaphrodite intestine (int) than in the male intestine. Somatic gonad signal in XO males appears localized to seminal vesicle and vas deferens cells. (C-E) XX emo-1(ozl) hermaphrodites showing signal only slightly above background in diakinesis-stage oocytes and undifferentiated germ cells. The young adult gonad (C) contains no endomitotic oocytes, whereas the slightly older gonad (D and E) contains many endomitotic oocytes (arrows),some of which (white arrowheads)show emo-1 signal, emo-1 signal in emo-1(ozl) intestines is predominately nuclear (blackarrowheads). | |||
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Name | FigO.jpg | ||||
Depict | Expr_pattern | Expr1565 | |||
Anatomy | WBbt:0005337 | ||||
WBbt:0005772 | |||||
WBbt:0005784 | |||||
WBbt:0005785 | |||||
WBbt:0006797 | |||||
WBbt:0006870 | |||||
Cellular_component | GO:0005634 | ||||
Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. The user must not use the Work for commercial purposes. If the user shares, alters, transforms, or builds upon the Work, the user may distribute the resulting work only under the same license the user received from <Publisher_URL>. | |||
Publication_year | 1996 | ||||
Article_URL | DOI | id | 10.1083/jcb.134.3.699 | ||
Journal_URL | TheJournalofCellBiology | ||||
Publisher_URL | TheRockefellerUniversityPress | ||||
Reference | WBPaper00002519 |