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WBPicture0000011607DescriptionFigure 4. vab-7::lacZ expression. (A-D) Embryos containing an eIs24, an integrated vab-7::lacZ fusion gene (plasmid pJA15 in Fig. B), stained for β-galactosidase activity using X-gal. Anterior, left. (A) 100-cell stage; (BI -200 cells; (C) -400 cells. (D) Embryo during early morphogenesis 1.5-fold stage. Arrowheads point to stained muscle cells; the majority are dorsal, located at the periphery. The arrow points to hypodermal cell staining at the posterior. No staining is detectable after the early L1 stage. Because vab-7 RNA is expressed in L2 larvae (Fig. 3A), the vab7::IacZ construct may lack some regulatory elements. (E) Cartoon showing cells that express vab-7::lacZ near the end of morphogenesis. Most cells that stain are descended from the C blastomere: nine dorsal and three ventral muscles on each side as well as some dorsal hypodermal cells (e.g., Cpapaa and Cpapap). In addition, two hypodermal cells produced by the ABblastomere that form the hyp10 syncytium also express the fusion gene.
NameFigD.jpg
DepictExpr_patternExpr93
AnatomyWBbt:0003675
WBbt:0005733
AcknowledgmentTemplateWormBase wishes to thank <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
Publication_year1996
Article_URLDOIid10.1101/gad.10.9.1120
Journal_URLGenes&Development
Publisher_URLColdSpringHarborLabPress
ReferenceWBPaper00002462