WormBase Tree Display for Picture: WBPicture0000011588

WBPicture0000011588 Description: Figure 4. Northern blot of total C.elegans RNA. Fifteen micrograms of total RNA, prepared from wild-type hermaphrodite (lanes A-G and I)or him-8(e1489) XX and XO (laneH) worms synchronized at each developmental stage (embryo, first to fourth larval (L1 stages,and adult), was loaded in each lane. Following electrophoresis and capillary transfer, the blot was hybridized to an antisense riboprobe prepared from a fem-2 cDNA clone. The top panel shows the resulting autoradiogram. The bottom panel shows the ethidium bromide-stained gel before Northern transfer to show relative loading. Lanes A, G, and H are embryo RNA. Lane B is L1, C is L2, D is L3, E is L4, and F and I are adult RNA. The blot in panel B was hybridized with a probe of higher specific activity than the blot in panelA to allow more sensitive detection of the embryonic mRNA.
  Name: FigH.jpg
  Depict: Expr_pattern: Expr1554
  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article <Article_URL>. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 1995
    Article_URL: DOI id 10.1091/mbc.6.9.1159
    Journal_URL: MolecularBiologyoftheCell
    Publisher_URL: TheAmericanSocietyForCellBiology
  Reference: WBPaper00002243