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WBPicture0000011584DescriptionFigure 5. Distribution of PAR-1 in the Gonad and Early Embryolmmunofluorescence image of gonads and embryos showing the distribution of the anti-PAR-l antibody (green) and nuclei (blue). (A) lmmunofluorescence image of a wild-type gonad showing strong fluorescence at the periphery of newly forming oocytes. Although we detect signal around the periphery of these young oocytes, the outer membrane signal is faint and is not apparent in this reproduction. Abbreviations: MN, meiotic nuclei; NFO, newly forming oocytes; MO, mature oocytes.(B) Double exposure of the same gonad shown in (A), showing the localization of anti-PAR-l relative to nuclei. (C) Double exposure showing absence of peripheral staining in par-1(it86) mutant gonad.(D-E) Wild-type 1- and 2-cell embryos, respectively, showing strong peripheral fluorescence in the posterior. (F-G) Mutant 1- and 2-cell par-l(lw39) embryos showing the absence of posterior peripheral staining and the reduction of cytoplasmic staining. The scale bar in (A)-(C) is 32 um; in (D)-(G), 8 um.
NameFig2.jpg
DepictExpr_patternExpr578
AnatomyWBbt:0005175
WBbt:0007028
AcknowledgmentTemplateReprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
Publication_year1995
Article_URLDOIid10.1016/0092-8674(95)90082-9
Journal_URLCell
Publisher_URLElsevier
ReferenceWBPaper00002189