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| WBPicture0000011580 | Description | Figure 7. Immunocytochemical analysis of whole-mount embryos using anti-bpat- 3cyto. Embryos are oriented with the head to the left. All embryos except those shown in E and G were colabeled with the monoclonal antibody MH27, which recognizes a desmosomal component at the apical surface of hypodermal and a subset of pharyngeal cells. The pattern of MH27 staining, which forms a grid-like pattern, can be seen in H. (A) Dorsal view of staining in a comma stage embryo, showing bpat-3 staining in developing muscle quadrants. (B) Same embryo as in A, mid-focal plane of embryo, showing bpat-3 staining in a subset of cells comprising the pharynx, tentatively identified as the marginal cells. (C) Dorsal view of a 1.5-fold embryo,showing bpat-3 staining in the dorsal muscle quadrants. (D) Same embryo as in C, mid-focal plane of embryo, showing staining in the pharynx, again tentatively identified as the marginal cells. (E) Three-fold embryo, showing bpat-3 staining in the anal depressor muscle. bpat-3 staining in body wall muscles is out of focus. Staining in the intestine is most likely independent of bpat-3 expression as it was also detected in pat-3(rh54) embryos (see Fig. 5 H) (F) Threefold embryo, right side, showing Bpat-3 staining in the right side anterior coelomo- cytes. (G) Threefold embryo, showing bpat-3 staining in a neuronal cell body and process located laterally in the anterior portion of the embryo, with the process extend- ing anteriorly. This neuron has been tentatively identified as the touch neuron ALM (see text). (H) Embryos stained with anti-bpat-3cyto and preincubated with 10 ug/ml of the cytoplasmic peptide show no detectable staining. Embryos were colabeled with MH27 to ensure that the animals had been permeabilized; the pattern of MH27 staining is shown for comparison with the other figures. Bars: (A-F and H) 5 um; (G) 10 um. | |||
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| Depict | Expr_pattern | Expr1545 | |||
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| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. The user must not use the Work for commercial purposes. If the user shares, alters, transforms, or builds upon the Work, the user may distribute the resulting work only under the same license the user received from <Publisher_URL>. | |||
| Publication_year | 1995 | ||||
| Article_URL | DOI | id | 10.1083/jcb.129.4.1127 | ||
| Journal_URL | TheJournalofCellBiology | ||||
| Publisher_URL | TheRockefellerUniversityPress | ||||
| Reference | WBPaper00002179 |
