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| WBPicture0000011208 | Description | FIG. 4. Northern blot analysis. Total cellular RNA (20-25 ug) isolated from embryo (A) and various stages of nematode development (B), were denatured, separated on a 1% agarose-formaldehyde gel, and blotted onto a nylon membrane (Sambrook et al., 1989). After hybridization with the 761-bp PCR fragment (A), the 2.8-kb insert of the cDNA clone pCef6A (BI), or the 3-kb actin gene 1 insert of pCeA7 (BII), filters were washed as described in Materials and Methods and exposed to X-ray film for 5 hr (panel BI) or overnight at -70 C using Dupont Cronex intensifying screens. Lower panel. Ethidium bromide-stained gel prior to blotting. E, embryo; L1 to L4, larvae; D, dauer larva; A, young adult; M, size markers. Sizes of marker bands are given in kilobases. | |||
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| Name | FigE.jpg | ||||
| Depict | Expr_pattern | Expr1390 | |||
| Acknowledgment | Template | Reprinted with permission from <Journal_URL>, published by <Publisher_URL>, <Publication_year>. | |||
| Publication_year | 1991 | ||||
| Article_URL | DOI | id | 10.1089/dna.1991.10.603 | ||
| Journal_URL | DNAandCellBiol | ||||
| Publisher_URL | MaryAnnLiebertInc | ||||
| Reference | WBPaper00001472 |
