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| WBPicture0000011109 | Description | Figure 4. Expression of an unc-2::GFP fusion construct in transgenic animals. A, GFP fluorescence exhibited by a typical transgenic L3 larva (left side view of animal). Expression is observed in most sensory neurons in the head (filled arrowhead), as well as a subset of motor neurons in the ventral nerve cord (VNC, double arrowhead). Expression of the GFP reporter is also observed in the interneurons SDQL and SDQR (not in focus) and the touch receptors including ALM and PVM. B, Right side view showing expression in the SDQR interneuron and the touch cells AVM and ALM. C, Confocal images of the head region of an L1 larva. The left image is a composite confocal z-series of the entire head showing all GFP fluorescent cells, whereas the single slice on right shows expression of the reporter construct in a single AWC sensory neuron cell body. Expression of unc-2::GFP was observed in both sister AWC cells, although for clarity only one side of the head is shown. D, Expression of unc-2::GFPin the HSN (arrow) and VC neurons that control egg-laying. The cell bodies of the VC neurons are located in the ventral nerve cord and are not in the same focal plane. Processes extending from the more anterior VCs that innervate the vulval muscles are indicated by the star (*). E, Expression of unc-2::GFP in the pre-anal, dorsal-rectal, and lumbar ganglia of the tail. GFP fluorescence in the DVB neuron (arrowhead) which is required for defecation is indicated. The barbed arrow in A and the triangle in D represent the position of the vulva. In all panels, the anterior of the animals is positioned to the left, and the dorsal side is topmost. | |
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| Name | F4.large.jpg | ||
| Depict | Expr_pattern | Expr2616 | |
| Anatomy (15) | |||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |
| Publication_year | 2003 | ||
| Journal_URL | TheJournalofNeuroscience | ||
| Publisher_URL | SocietyofNeuroscience | ||
| Reference | WBPaper00006005 |
