WormBase Tree Display for Picture: WBPicture0000011106
expand all nodes | collapse all nodes | view schema
| WBPicture0000011106 | Description | Figure 1. UNC-49 GABA receptors form postsynaptic clusters at neuromuscular junctions. A, Immunostaining of UNC-49. Young wild-type adults were immunolabeled with rabbit anti-UNC-49 antibody. UNC-49 receptors are present in the nerve ring (open arrowheads) and along ventral and dorsal nerve cords. In this panel, as well as in all following figures, anterior is to the left and ventral is down.B, High magnification of the ventral nerve cord reveals that UNC-49 clusters into individual puncta. C, Visualization of GABAergic varicosities in the ventral nerve cord of a transgenic worm expressing in GABAergic neurons the synaptic vesicle protein synaptobrevin SNB-1 fused to CFP. Note that some SNB-1-CFP accumulates in motor neuron soma (arrows).D, In the same transgenic animal, muscle GABA receptors are visualized by expressing UNC-49 fused to YFP. UNC-49-YFP fluorescence is detected in muscle cells in a punctate pattern along the nerve cord. Note that some weak UNC-49-YFP signal outlines muscle cell membranes. E, Merging SNB-1-CFP and UNC-49-YFP signals shows that UNC-49 clusters are localized opposite GABA release sites (inset, detail of ventral cord labeling). Scale bars, 20 um (for all images). | |
|---|---|---|---|
| Name | F1.large.jpg | ||
| Depict | Expr_pattern | Marker2 | |
| Anatomy | WBbt:0005829 | ||
| WBbt:0006749 | |||
| WBbt:0006750 | |||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |
| Publication_year | 2003 | ||
| Journal_URL | TheJournalofNeuroscience | ||
| Publisher_URL | SocietyofNeuroscience | ||
| Reference | WBPaper00005798 |
