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WBPicture0000011076 | Description | Figure 5. UNC-49B and UNC-49C are coexpressed and colocalized. A, Structure of UNC-49:: GFP transgenes. The left panel shows the site at which GFP was inserted, in frame, into the unc-49 rescuing fragment. Vertical bars represent transmembrane domains. The right panel shows the subunits that are produced by the transgene. GFP indicates subunits tagged with GFP; + indicates wild-type subunits; - indicates inactivated subunits. B, Fluorescence micrographs of UNC-49B:: GFP transgenic worms. Left panel, Bright GFP fluorescence is visible in a punctate pattern along the nerve cord, where neuromuscular junctions are located. Fainter GFP fluorescence is also visible outlining the muscle cell bodies (lens-shaped bodies beneath the nerve cord) and muscle arms (narrow processes extending from the muscle cell bodies to the nerve cord).Right panel, Tail region of an UNC-49B:: GFP worm showing bright fluorescence in the sphincter muscle. C, Fluorescence micrographs of UNC-49C:: GFP transgenic worms. The pattern of fluorescence is similar to that observed in the UNC-49B:: GFP transgenic animals in the body wall muscles and nerve cord (left panel). However, no fluorescence is visible in the sphincter muscle (right panel). | |
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Name | F5.large.jpg | ||
Depict | Expr_pattern | Expr1283 | |
Anatomy | WBbt:0005798 | ||
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WBbt:0005829 | |||
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Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |
Publication_year | 1999 | ||
Journal_URL | TheJournalofNeuroscience | ||
Publisher_URL | SocietyofNeuroscience | ||
Reference | WBPaper00003576 |