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WBPicture0000011076DescriptionFigure 5. UNC-49B and UNC-49C are coexpressed and colocalized. A, Structure of UNC-49:: GFP transgenes. The left panel shows the site at which GFP was inserted, in frame, into the unc-49 rescuing fragment. Vertical bars represent transmembrane domains. The right panel shows the subunits that are produced by the transgene. GFP indicates subunits tagged with GFP; + indicates wild-type subunits; - indicates inactivated subunits. B, Fluorescence micrographs of UNC-49B:: GFP transgenic worms. Left panel, Bright GFP fluorescence is visible in a punctate pattern along the nerve cord, where neuromuscular junctions are located. Fainter GFP fluorescence is also visible outlining the muscle cell bodies (lens-shaped bodies beneath the nerve cord) and muscle arms (narrow processes extending from the muscle cell bodies to the nerve cord).Right panel, Tail region of an UNC-49B:: GFP worm showing bright fluorescence in the sphincter muscle. C, Fluorescence micrographs of UNC-49C:: GFP transgenic worms. The pattern of fluorescence is similar to that observed in the UNC-49B:: GFP transgenic animals in the body wall muscles and nerve cord (left panel). However, no fluorescence is visible in the sphincter muscle (right panel).
NameF5.large.jpg
DepictExpr_patternExpr1283
AnatomyWBbt:0005798
WBbt:0005813
WBbt:0005829
WBbt:0006750
AcknowledgmentTemplateWormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
Publication_year1999
Journal_URLTheJournalofNeuroscience
Publisher_URLSocietyofNeuroscience
ReferenceWBPaper00003576