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WBPicture0000011065 | Description | Figure 2. Expression of snb-1 in theC. elegans nervous system. A-F, Whole worms fixed and stained with a-SNB-1, a-RAB-3, and a-UNC-64 (syntaxin) primary antibodies and visualized with FITC- or Cy3-conjugated antibodies. A, Lateral view of the head region of a wild-type adult hermaphrodite showing SNB-1 immunoreactivity in the nerve ring, pharyngeal nervous system, and ventral and dorsal nerve cords. B, Ventral view of the tail region of a wild-type adult hermaphrodite showing SNB-1 immunoreactivity in the ventral nerve cord. C, D, Ventral view of the midsection of a wild-type adult hermaphrodite showing colocalization of SNB-1 (C) and RAB-3 (D) immunoreactivity in the ventral nerve cord.E, Ventral view of the head of SNB-1 immunoreactivity in an adult snb-1(md247) mutant. The SNB-1 mutant protein remains limited to the synaptic regions of the SAB neurons.F, Similar view of UNC-64 syntaxin immunoreactivity in a wild-type animal reveals all processes in the head. G, H, Transgenic jsEx96 animals expressing thep snb-1:: lacZreporter construct pSB111 stained for b-galactosidase activity, using X-gal. lacZ contains an SV40 nuclear localization signal. G, Lateral view of the head region of an adult hermaphrodite showing staining neurons in the head ganglia and pharynx.H, Lateral view of the vulval region of an adult hermaphrodite showing staining in the motor neurons in the ventral cord and two neurons in the deirid sensillum. | |
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Name | F2.large.jpg | ||
Crop | Crop_picture | WBPicture0000011066 | |
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Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |
Publication_year | 1998 | ||
Journal_URL | TheJournalofNeuroscience | ||
Publisher_URL | SocietyofNeuroscience | ||
Reference | WBPaper00002986 |