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WBPicture0000011058DescriptionFigure 3. Serial optical sections showing expression of the unc-29 promoter-driven unc-29:: gfp fusion LJH5 in N2 wild type and in the unc-29(x29) mutant strain. Confocal microscopic sections through the head region of a wild-type and an x29 mutant animal were accumulated as described in Materials and Methods. Each picture represents a successive 3 um thickness of the head. A-E, Wild type. Arrows in A point to fluorescence in head muscles. Fluorescence is seen more intensely inside the same muscles in B and also within a body muscle (arrow). In E, a muscle process entering the central neuropil next to the isthmus of the pharynx is stained (arrow). Stain is also accumulated in a neuronal cell body (arrowhead). F-J, unc-29(x29) mutant. Less staining is seen overall, and the stain is relatively concentrated in head muscle processes and nerve cords with continued neuronal staining. In F, process from anterior head muscles stain (arrowhead) and punctate staining is seen in a nerve cord (arrow). In H, the arrow points to a brightly staining neuronal cell body with a process running to the central neuropil, appearing as a hazy band in the center. In I, arrows point to brightly stained areas in the central neuropil immediately adjacent to the isthmus of the pharynx that appear to be associated with muscle processes running into the neuropil at this point. The same region is stained in the center in J, with two neuronal cell bodies below the center.
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AcknowledgmentTemplateWormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
Publication_year1997
Journal_URLTheJournalofNeuroscience
Publisher_URLSocietyofNeuroscience
ReferenceWBPaper00002860