WormBase Tree Display for Picture: WBPicture0000011007

WBPicture0000011007 Description: Figure 6. CDF-2 was expressed in intestinal cells and localized to membrane-bound vesicles. Transgenic animals expressing CDF-2::GFP were cultured on NGM with live E. coli. Live adults were immobilized and mounted (A-D). The inset in D is a 2.4 magnification of the boxed region. Mixed-stage worms were fixed and stained with an anti-GFP antibody (E-U). Differential interference contrast images display organism morphology (A, E, I, M, and Q), green displays CDF-2::GFP and autofluorescence (B) or only CDF-2::GFP (F, J, N, R, and U), red displays autofluorescence (C, G, K, O, and S), yellow displays overlap between CDF-2::GFP and autofluorescence (D), and blue displays nuclear morphology using DAPI (H, L, P, and T). Red in C shows a punctate pattern of autofluorescence in intestinal cells, whereas red in G, K, O, and S shows that there is no specific localization of autofluorescence following fixation, and exposure times for red in these panels were at least fivefold longer than for green. CDF-2::GFP was first detected during embryogenesis at the E16-E20 stage (F), and expression persisted throughout embryogenesis (J). CDF-2::GFP was expressed in a punctate pattern in intestinal cells during all larval stages (N) and in adults (R). Puncta appear to be membrane-bound vesicles (arrows in U).
  Name: F6.large.jpg
  Depict: Expr_pattern: Expr8721
    Anatomy: WBbt:0005772
    Cellular_component: GO:0012506
  Acknowledgment: Template: WormBase thanks the journal <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>
    Publication_year: 2009
    Article_URL: DOI id 10.1534/genetics.109.103614
    Journal_URL: Genetics
    Publisher_URL: GSA
  Reference: WBPaper00033166