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WBPicture0000010847DescriptionFig. 2. Imaging synaptobrevin in intact animals. (Aa) The N-terminal tag places GFP (NGFP-SNB) in the cytoplasm. (Ab) Motor neurons expressing a C-terminal pHluorin-tagged synaptobrevin (SpH) that places the fluorophore in the vesicle lumen. (Scale bars, 5 um.) (Ba) Expression of SpH in the ventral cord. Neuromuscular junctions are located on the bottom cord (*). (Bb) Expression of UNC-10 RIM1::RFP in the ventral cord. (Bc) Colocalization of SpH (green) and UNC-10 (red). (C) A line scan of SpH in a wild-type animal. Peaks (red arrowheads) and baseline (dashed line) were located by using automated software, as described in Supporting Methods.
NameF1.jpg
DepictExpr_patternExpr4216
AnatomyWBbt:0005409
WBbt:0005829
Cellular_componentGO:0005737
AcknowledgmentTemplateReprinted with permission from <Journal_URL> <Article_URL>. Copyright (<Publication_year>), <Publisher_URL>.
Publication_year2006
Article_URLDOIid10.1073/pnas.0600784103
Journal_URLProcNatlAcadSciUSA
Publisher_URLNationalAcademyofSciencesUSA
ReferenceWBPaper00027755