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| WBPicture0000010818 | Description | Figure 1. Matefin in C. elegans and its interaction with Ce-lamin. (A) A schematic diagram of full-length matefin, drawn approximately to scale. Bars on top indicate the positions of the gk199 deletion, the peptides used to prepare serum 3663 and 3665 antibodies against matefin, and the anti-erbB-2 IgG domain. T1 and T2, putative transmembrane regions 1 and 2, respectively; CC, coiled-coil domain; SUN, SUN domain. (B) Western blot of crude protein extract of mixed stage C. elegans animals probed with preimmune (P) or immune (I) serum 3665. (C) Indirect immunofluorescence double-staining of embryos by using antibodies specific for Ce-lamin plus either the N terminus (serum 3663, Lower) or C terminus (serum 3665, Upper) of endogenous matefin. Please note that although both embryonic and adult nuclei are stained with Ce-lamin, only the embryonic nuclei are stained with matefin (Upper). (Bars, 10 μm.) (D) Immunogold TEM localization of the matefin N terminus (serum 3663) on peripheral chromatin in detergent-extracted nuclei. Fragments of remaining nuclear outer membrane (OM) and inner membrane (IM) are indicated. Arrows indicate the position of the 6-nm gold labeling. (Bar, 100 nm.) (E) Equal amounts of protein, as determined by Bradford assay, were resolved by SDS/PAGE, transferred to nitrocellulose, stained with Ponceau S, and probed with either 35S-matefin or 35S-Ce-lamin. Please note that Ponceau S staining does not always correspond to Bradford assay results. Numbered gel lanes contain the following polypeptides: 1, Ce-emerin (residues 1-130); 2, the C-terminal globular domain of Ce-lamin (residues 440-566); 3, Ce-MAN1 (residues 400-500); 4, BSA; 5, Ce-lamin; 6, matefin; 7, matefin N-terminal domain (residues 1-111); 8, matefin C-terminal domain (residues 129-473). | |||
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| Name | F1.large.jpg | ||||
| Depict | Expr_pattern | Expr2947 | |||
| Acknowledgment | Template | Reprinted with permission from <Journal_URL> <Article_URL>. Copyright (<Publication_year>), <Publisher_URL>. | |||
| Publication_year | 2004 | ||||
| Article_URL | DOI | id | 10.1073/pnas.0307880101 | ||
| Journal_URL | ProcNatlAcadSciUSA | ||||
| Publisher_URL | NationalAcademyofSciencesUSA | ||||
| Reference | WBPaper00006526 |
