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WBPicture0000010808 | Description | Figure 2. PQE-1B and PQE-1C are nuclear proteins. Subcellular localization of PQE-1B:GFP and PQE-1C:GFP was examined by using the osm-10 promoter to drive transgene expression. The PQE-1B:GFP and PQE-1C:GFP fusion proteins were detected in the nucleus of the ASH neurons of live animals (A and D, respectively). The cell bodies of the corresponding ASH neurons were visualized by dye filling with DiD (B and E, respectively). PQE-1B:GFP and PQE-1C:GFP were detected in the ASH nuclei of Bowin's fixed animals (35) by using affinity-purified antisera that recognize the C and N termini of PQE-1 (C and F, respectively). PQE-1B:GFP and PQE-1C:GFP are still localized to the nucleus in animals expressing Htn-Q150 (not shown). (Bar = 0.5 um.) | |||
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Name | F2.large.jpg | ||||
Depict | Expr_pattern | Expr8163 | |||
Cellular_component | GO:0005634 | ||||
Acknowledgment | Template | Reprinted with permission from <Journal_URL> <Article_URL>. Copyright (<Publication_year>), <Publisher_URL>. | |||
Publication_year | 2002 | ||||
Article_URL | DOI | id | 10.1073/pnas.262544899 | ||
Journal_URL | ProcNatlAcadSciUSA | ||||
Publisher_URL | NationalAcademyofSciencesUSA | ||||
Reference | WBPaper00005585 |