WormBase Tree Display for Picture: WBPicture0000010759

WBPicture0000010759 Description: Figure 1. avr-14 encodes a subunit of an ivermectin-sensitive GluCl. (A) The mutations in alternatively spliced transcripts of avr-14. The exons encoding the extracellular domain (black) are shared by both transcripts. The exons encoding the transmembrane domains (stippled) are produced by alternatively spliced exons (white in AVR-14A and gray in AVR-14B). The region surrounding the conserved valine (bold) that is mutated to a glutamate in the ad1302 allele (resulting from a T to A mutation in the second base of the V60 codon) is shown lined up with the corresponding region in other GluCl subunits and in the rat glycine and gamma-aminobutyric acid (GABA) type A-b channel subunits (29, 30). The ad1305 mutation results from an A to T change in the first base of the R387 codon. (B) Electrophysiology of oocytes expressing AVR-14B/GluCla3B. Oocytes were injected with RNA transcribed from AVR-14B/GluCla3B and were voltage clamped; 10 mM glutamate or 10 uM ivermectin (in 1% DMSO) were applied during the periods of time indicated by the bars. (C) Expression of an avr-14 reporter construct. A GFP reporter construct was generated by fusing GFP in-frame to the sixth exon of avr-14. GFP fluorescence of an L4 worm is shown. The identified neuronal cell bodies (arrows) are indicated by their three-letter code. Arrowheads indicate ventral cord neurons. Multiple fluorescent neurons in the ring ganglia are indicated by the bracket. (Scale bar indicates 50 um).
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  Crop: Crop_picture: WBPicture0000010760
  Acknowledgment: Template: Reprinted with permission from <Journal_URL> <Article_URL>. Copyright (<Publication_year>), <Publisher_URL>.
    Publication_year: 2000
    Article_URL: DOI id 10.1073/pnas.97.6.2674
    Journal_URL: ProcNatlAcadSciUSA
    Publisher_URL: NationalAcademyofSciencesUSA
  Reference: WBPaper00003954