WormBase Tree Display for Picture: WBPicture0000010745

WBPicture0000010745 Description: Figure 2. (A) Discrete fluorescent foci are observed in the embryonic gut nuclei of strain JM73, i.e., embryos homozygous for a transgenic array containing the full rescuing elt-2::GFP construct pJM86. Three representative embryos (left to right) at the 8E cell stage are shown. (Upper) The stack of serially collected 400-nm optical sections was projected without further manipulation onto a single plane. (Lower) The same images were first subjected to digital deconvolution and then projected onto a single plane. The arrows point to an example of two distinct fluorescent foci within a single gut nucleus. The scale bar represents 10 um. (B) Discrete fluorescent foci are not observed in gut nuclei of embryos transgenic for the construct pJM67, i.e., a GFP fusion to elt-2 that removes the DNA binding domain (see Fig. 1c). Three typical 8E cell stage embryos are shown; all nuclei of the eight gut cells are intensely fluorescent but no discrete foci are observed. Shown are image stacks before (Upper) and after (Lower) digital deconvolution. (C) Single fluorescent foci are observed in gut nuclei of embryos heterozygous for the transgenic array containing the full rescuing elt-2::GFP construct pJM86. As described in the text, male worms of strain JM73 were crossed with wild-type hermaphrodites; fluorescent embryos in the next generation therefore must be heterozygous cross progeny and were imaged as usual at the 8E cell stage. Projected images of three typical embryos are shown, either before (Upper) or after (Lower) digital deconvolution. (D) An extra fluorescent focus appears in the gut cells of embryos carrying one copy of a transgenic array containing pJM86 (i.e., the full rescuing elt-2::GFP construct) and a second independent transgenic array containing pJM72 (i.e., the elt-2 promoter fused to a lacZ reporter gene; see Fig. 1d). The images represent the projected image stack without (Upper) or with (Lower) digital deconvolution. The arrow points to a gut nucleus in which one fluorescent focus is condensed and the second is extended or fibrillar. For each of the above experimental situations, full data sets were collected for at least 25 embryos.
  Name: F2.large.jpg
  Depict: Expr_pattern: Expr1216
    Anatomy: WBbt:0005772
    Cellular_component: GO:0005634
  Acknowledgment: Template: Reprinted with permission from <Journal_URL> <Article_URL>. Copyright (<Publication_year>), <Publisher_URL>.
    Publication_year: 1999
    Article_URL: DOI id 10.1073/pnas.96.21.11883
    Journal_URL: ProcNatlAcadSciUSA
    Publisher_URL: NationalAcademyofSciencesUSA
  Reference: WBPaper00003733