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| WBPicture0000010697 | Description | Figure 4. Dauer formation in daf-2(e1370) mutant animals is associated with an increase in autophagy in lateral hypodermal seam cells. (A) GFP::LGG-1 expression in the seam cells of wild-type N2 and daf-2(e1370) L3 larvae grown at 15C and 25C. The arrow shows representative GFP-positive punctate areas that label preautophagosomal and autophagosomal structures. (B) Quantitation of GFP::LGG-1-positive punctate areas in the seam cells of wild-type N2 and daf-2(e1370) animals grown at 15C and 25C (mean + SEM for at least 50 seam cells from 10 different animals). (C to I) Representative electron micrographs of lateral hypodermal seam cells in a wild-type N2 control animal in (C) the L2 larval stage and (D) the L3 larval stage; a daf-2(e1370) animal grown for (E) 1 day at 25C and [(F) to (H)] 2 days at 25C; and (I) a daf-2(e1370); bec-1 (RNAi) animal grown for 2 days at 25C. In (F), arrowheads denote representative vacuolar structures in the late stages of the autophagolysosomal pathway; the white arrowhead denotes structure that is shown at higher magnification in (H); the arrow denotes the dauer cuticle. (G) A representative early autophagosome and (H) a multilamellar body/autolysosome are shown in a seam cell of a daf-2(e1370) dauer animal. Scale bars, 1 uM [(C) to (F) and (I)]; 0.1 uM [(G) and (H)]. | |||
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| Name | F4.large.jpg | ||||
| Crop | Crop_picture | WBPicture0000010698 | |||
| Acknowledgment | Template | From <Article_URL>. Reprinted with permission from <Publisher_URL>. Readers may view, browse, and/or download material for temporary copying purposes only, provided these uses are for noncommercial personal purposes. Except as provided by law, this material may not be further reproduced, distributed, transmitted, modified, adapted, performed, displayed, published, or sold in whole or in part, without prior written permission from the publisher. | |||
| Publication_year | 2003 | ||||
| Article_URL | DOI | id | 10.1126/science.1087782 | ||
| Journal_URL | Science | ||||
| Publisher_URL | AAAS | ||||
| Reference | WBPaper00006070 |
