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| WBPicture0000010678 | Description | Figure 4. The onset of expression in the pharynx can be altered by changes in the affinity of TRTTKRY elements for PHA-4. (A) Diagrams of promoter fragments used to drive expression of a GFP::HIS2B reporter. Vertical lines represent TRTTKRY elements that were not mutated in these experiments or whose relative affinity for PHA-4 has not been determined in vitro. Triangles represent TRTTKRY elements mutated in these experiments: black, gray, and open triangles represent high- (TGTTTGC or TGTTGAC), medium- (TGTTTGT), and low- (TATTTGT) affinity PHA-4 binding sequences, respectively. 'Onset' refers to the developmental stage at which expression of GFP in the pharynx is first detected (22). (B through M) Embryos expressing GFP; brackets indicate the developing pharynx. (B through D) Pharyngeal expression of the -196 T05E11.3::GFP reporter containing the wild-type predicted PHA-4 site [TGTTTGC; shown in (A)iii]. (E through G) Pharyngeal expression of the -196 T05E11.3::GFP reporter containing a mutated site [TGTTTGT; shown in (A)iii]. (H through J) Pharyngeal expression of the -535 D2096.6::GFP reporter containing the wild-type predicted PHA-4 site [TGTTTGT; shown in (A)ii]. (K through M) Expression of the -535 D2096.6::GFP reporter containing a mutated site [TGTTTGC; shown in (A)ii]. | |||
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| Name | F4.large.jpg | ||||
| Crop | Crop_picture | WBPicture0000010679 | |||
| WBPicture0000010680 | |||||
| Acknowledgment | Template | From <Article_URL>. Reprinted with permission from <Publisher_URL>. Readers may view, browse, and/or download material for temporary copying purposes only, provided these uses are for noncommercial personal purposes. Except as provided by law, this material may not be further reproduced, distributed, transmitted, modified, adapted, performed, displayed, published, or sold in whole or in part, without prior written permission from the publisher. | |||
| Publication_year | 2002 | ||||
| Article_URL | DOI | id | 10.1126/science.1065175 | ||
| Journal_URL | Science | ||||
| Publisher_URL | AAAS | ||||
| Reference | WBPaper00005056 |
