WormBase Tree Display for Picture: WBPicture0000010675
expand all nodes | collapse all nodes | view schema
| WBPicture0000010675 | Description | Figure 1. Pharyngeal expression requires TRTTKRY elements. (A) Diagrams of promoter fragments used to drive expression of a GFP::HIS2B reporter (13). Triangles represent TRTTKRY elements on the top or bottom strand, and X's indicate eliminated TRTTKRY elements. Numbers indicate the 5' end relative to the predicted start codon of each gene. 'Pharynx expression' is the relative strength of pharyngeal GFP at its peak in embryogenesis, assessed visually. For M05B5.2 andmyo-2, notes in parentheses indicate the relative onset of GFP expression (22).ceh-22, myo-2, andtph-1 are known to be expressed in the pharynx (14, 32-34). (B throughE) Embryos and larvae expressing GFP. Anterior is left, and dorsal is up. Brackets indicate pharyngeal cells. (B) Pharyngeal expression of the -196 T05E11.3::GFP reporter with a wild-type predicted PHA-4 site [TGTTTGC; shown in (A)i]. (C) GFP expression of the -196 T05E11.3::GFP reporter with a disrupted site (TGGATCC) is faintly visible in cells outside the pharynx [shown in (A)i]. (D) Pharyngeal expression of the -535 D2096.6::GFP reporter containing the wild-type predicted PHA-4 site [TGTTTGT; shown in (A)ii]. (E) GFP expression of the -535 D2096.6::GFP reporter with a disrupted site [TCCGCGG; shown in (A)ii]. Faint GFP fluorescence is detected in epidermal and gut (arrowhead) cells. | |||
|---|---|---|---|---|---|
| Name | F1.large.jpg | ||||
| Crop | Crop_picture | WBPicture0000010676 | |||
| WBPicture0000010677 | |||||
| Acknowledgment | Template | From <Article_URL>. Reprinted with permission from <Publisher_URL>. Readers may view, browse, and/or download material for temporary copying purposes only, provided these uses are for noncommercial personal purposes. Except as provided by law, this material may not be further reproduced, distributed, transmitted, modified, adapted, performed, displayed, published, or sold in whole or in part, without prior written permission from the publisher. | |||
| Publication_year | 2002 | ||||
| Article_URL | DOI | id | 10.1126/science.1065175 | ||
| Journal_URL | Science | ||||
| Publisher_URL | AAAS | ||||
| Reference | WBPaper00005056 |
