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| WBPicture0000010657 | Description | Figure 1. flp-1 deletion alleles. (A) Genetic position of flp-1 on the right arm of chromosome IV. (B) Genomic organization of flp-1. Top line: partial restriction map of the flp-1 genomic region. E, Eco RI; C, Cla I. Bottom line: Expanded genomic region to show organization of flp-1. Exons are indicated as boxes (peptide coding sequences are indicated in black), introns as lines; the site of the Tc1 insertion is indicated by an inverted triangle. Two deletions of the flp-1 gene were isolated by PCR screening of populations of Tc1 insertion strains for an imprecise Tc1 excision (13). The extent of the yn2(1.4 kbp) and yn4 (2.1 kbp) deletions are shown; both deletions remove upstream and coding regions. Primers used to screen for deletions are indicated by arrows. (C) Backcrossed yn2 and yn4 strains are homozygous deletion lines. Genomic DNA was isolated from backcrossedyn2 and yn4 animals, digested with Cla I, and Southern blotted. The position of the probe is shown in (B). Solid arrow indicates wild-type hybridizing fragment; double arrows indicate smaller hybridizing fragments resulting from the yn2 andyn4 deletions. No hybridizing fragment corresponding to the wild-type fragment is detected in DNA from homozygous yn2and yn4 animals. Molecular weight markers (kilobase pairs) are indicated to the left. (D) Expression offlp-1 in wild-type animals is detected only in anterior head neurons. Transgenic animals were stained for beta-galactosidase activity to visualize expression of flp-1-lacZ reporter constructs (10). Scale bar, 25 um. | |||
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| Name | F1.large.jpg | ||||
| Crop | Crop_picture | WBPicture0000010658 | |||
| Acknowledgment | Template | From <Article_URL>. Reprinted with permission from <Publisher_URL>. Readers may view, browse, and/or download material for temporary copying purposes only, provided these uses are for noncommercial personal purposes. Except as provided by law, this material may not be further reproduced, distributed, transmitted, modified, adapted, performed, displayed, published, or sold in whole or in part, without prior written permission from the publisher. | |||
| Publication_year | 1998 | ||||
| Article_URL | DOI | id | 10.1126/science.281.5383.1686 | ||
| Journal_URL | Science | ||||
| Publisher_URL | AAAS | ||||
| Reference | WBPaper00003193 |
