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| WBPicture0000010182 | Description | Figure 3. Preferential expression of clh-1 in hypodermal cells. Transgenic worms harboring (a), (b) pTO17-14 and (c) pTO17-13 were permeabilized and then stained with 5-bromo-4-chloro-3-indolyl-b-d-galactoside (Fire et al., 1990). (a) and (b) An anterior portion of a transgenic worm carrying pTO17-14. Staining is predominant in the nuclei of hypodermal cells, called seam cells (open arrowheads), which run along the sides. Each side of a worm is shown in (a) and (b) to indicate the bilateral orientation of these cells. (c) A transgenic worm carrying pTO17-13. lacZ staining was clearly detectable in hypodermal cells of the head and vulva (filled arrowhead) in addition to seam cells. Diffuse staining in the cytoplasm of the seam syncytia was observed possibly due to the high expression of the fusion protein, although the lacZ fusion protein had a nuclear localization signal. The scale bars represent 0.1 mm. | |||
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| Name | F3.jpg | ||||
| Depict | Expr_pattern | Expr978 | |||
| Anatomy | WBbt:0005373 | ||||
| WBbt:0005753 | |||||
| WBbt:0006748 | |||||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
| Publication_year | 1999 | ||||
| Article_URL | DOI | id | 10.1006/jmbi.1999.3241 | ||
| Journal_URL | JournalofMolecularBiology | ||||
| Publisher_URL | Elsevier | ||||
| Reference | WBPaper00003816 |
