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WBPicture0000010172 Description: Fig. 3. Changes in the expression of the C. elegans set-1 gene during development. (A) Northern blot analysis of set-1 expression during embryonic and larval development. Upper panel: RNA prepared at different developmental stages was hybridised with a genomic probe corresponding to set-1. Lower panel: The blot was stripped and re-hybridised with an actin loading control. The position of the actin mRNA (transcript size, 1269 nucleotides) is shown in the upper panel. High expression is observed in eggs and decreases in successive developmental stages. (B-G) Whole mount in situ hybridisation analysis of set-1 expression during development. Data kindly provided by Yuji Kohara (http://nematode.lab.nig.ac.jp/) for the yk179f12 cDNA. In situ hybridisations of embryos and larvae were carried out as described (Mochii et al., 1999); those for adult worms used an unpublished protocol described at http://helix.genes.nig.ac.jp/db/. A very high and ubiquitous expression at the early stages of development (B-D) decreases by the 1 1/2-fold stage (E) and becomes further restricted later during larval development. (F) At the L3-L4 stages, strong expression is seen at the site of the presumptive vulva (arrowhead), and weak expression can be observed in the developing gonad (arrow). (G) In young adults expression is also seen in the gonad (arrow).
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  Crop: Crop_picture: WBPicture0000010173
  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 2002
    Article_URL: DOI id 10.1016/S0378-1119(02)00671-6
    Journal_URL: Gene
    Publisher_URL: Elsevier
  Reference: WBPaper00005352