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| WBPicture0000010105 | Description | Fig. 3. phf-5::yfp expression pattern in strain EC611: unc-119(ed3) III; eeIs611[unc-119(wt) phf-5::yfp]. (A-D), (I-M), (R-S): Nomarski differential interference contrast. (E-H), (N-Q), (T-U): corresponding yellow epifluorescent images. (A) Embryo of 100-250 cells. (B-D) Comma-staged embryos. (I) Pretzel-staged embryo. (K) L1 larvae. (L) Middle body part of a L1 larvae at higher magnification. (M) Apical body part of a L1 larvae at higher magnification. (R) L3 larvae. (S) Adult worm. (E) EYFP-fluorescence in the nuclei of two neighboring single cells within the central embryo mass. (F, G) phf-5::yfp expression in early comma-staged embryos is extended to multiple cell types. (H) Arrow points to EYFP fluorescence in the region of the posterior (rectal) opening. (N) Arrow points to a dominant expression domain in the embryonic pharynx. (O) Arrow 1 points to intense EYFP signals in the pharynx, arrow 2 points to a EYFP fluorescence signal in a polyploid gut cell nucleus. Within this focal plane altogether 10 polyploid gut cell nuclei with strong phf-5::yfp expression are visualized. (P) Strong EYFP signals are present in the cell nuclei of polyploid gut cells (a) and in cell nuclei and cytoplasm of body wall muscle cells (c). (b) Autofluorescent gut granules. (Q) PHF-5::YFP distribution in the pharyngeal region shows a nuclear predominance while a lower cytoplasmatic distribution paints the pharyngeal tissue in a homogeneous manner. (T) EYFP fluorescence is present in the region of the anal muscle and in body wall muscles of L3 larvae. (U) phf-5::yfp expression is restricted to pharyngeal muscle structures in adult worms. | |||
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| Name | F3.jpg | ||||
| Depict | Expr_pattern | Expr2000 | |||
| Anatomy | WBbt:0003681 | ||||
| WBbt:0005772 | |||||
| WBbt:0005803 | |||||
| WBbt:0005813 | |||||
| Cellular_component | GO:0005634 | ||||
| GO:0005737 | |||||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>. | |||
| Publication_year | 2002 | ||||
| Article_URL | DOI | id | 10.1016/S0006-291X(02)02276-3 | ||
| Journal_URL | BiochemicalandBiophysicalResearchCommunications | ||||
| Publisher_URL | Elsevier | ||||
| Reference | WBPaper00005466 |
